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Please use this identifier to cite or link to this item: http://hdl.handle.net/1834/3354

Title: Estimates of DNA strand breakage in bottlenose dolphin (Tursiops truncatus)
Authors: Blanco, M.
Cruz, D.
Carro, S.
López, R.
Díaz, A.
Sánchez, L.
Santiago, L.
Guevara, C.
Sánchez, L.
Cuetara, E.B.
López, N.
ASFA Terms: DNA
Species
Issue Date: 2009
Citation: Genetics and Molecular Biology, 32(2). p. 367-372
Abstract: The analysis of DNA damage by mean of Comet or single cell gel electrophoresis (SCGE) assay has been commonly used to assess genotoxic impact in aquatic animals being able to detect exposure to low concentrations of contaminants in a wide range of species. The aims of this work were 1) to evaluate the usefulness of the Comet to detect DNA strand breakage in dolphin leukocytes, 2) to use the DNA diffusion assay to determine the amount of DNA strand breakage associated with apoptosis or necrosis, and 3) to determine the proportion of DNA strand breakage that was unrelated to apoptosis and necrosis. Significant intra-individual variation was observed in all of the estimates of DNA damage. DNA strand breakage was overestimated because a considerable amount (~29%) of the DNA damage was derived from apoptosis and necrosis. The remaining DNA damage in dolphin leukocytes was caused by factors unrelated to apoptosis and necrosis. These results indicate that the DNA diffusion assay is a complementary tool that can be used together with the Comet assay to assess DNA damage in bottlenose dolphins.
URI: http://hdl.handle.net/1834/3354
Related document: www.sbg.org.br
ISSN: 1415-4757
Appears in Collections:1. Artículos científicos

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