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Please use this identifier to cite or link to this item:
http://hdl.handle.net/1834/3085
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| Title: | Estimates of DNA strand breakage in bottlenose dolphin ( Tursiops truncatus) leukocytes measured with the Comet and DNA diffusion assays |
| Authors: | Díaz, A.
Carro, S.
Santiago, L.
Estévez, J.
Guevara, C.
Blanco, M.
Sánchez, L.
Sánchez, L.
López, N.
Cruz, D.
López, R.
Cuetara, E.B.
Fuentes, J.L. |
| Issue Date: | 2009 |
| Publisher: | Sociedade Brasileira de Genética |
| Citation: | Genetics and Molecular Biology, 32(2). p. 367-372 |
| Abstract: | The analysis of DNA damage by mean of Comet or single cell gel electrophoresis (SCGE) assay has been commonly
used to assess genotoxic impact in aquatic animals being able to detect exposure to low concentrations of contaminants
in a wide range of species. The aims of this work were 1) to evaluate the usefulness of the Comet to detect DNA
strand breakage in dolphin leukocytes, 2) to use the DNA diffusion assay to determine the amount of DNA strand
breakage associated with apoptosis or necrosis, and 3) to determine the proportion of DNA strand breakage that was
unrelated to apoptosis and necrosis. Significant intra-individual variation was observed in all of the estimates of DNA
damage. DNA strand breakage was overestimated because a considerable amount (~29%) of the DNA damage was
derived from apoptosis and necrosis. The remaining DNA damage in dolphin leukocytes was caused by factors unrelated
to apoptosis and necrosis. These results indicate that the DNA diffusion assay is a complementary tool that can
be used together with the Comet assay to assess DNA damage in bottlenose dolphins. |
| URI: | http://hdl.handle.net/1834/3085 |
| Related document: | www.sbg.org.br |
| Appears in Collections: | 1. Artículos científicos
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