Prospholipid and fatty acid composition of two brachyuran decapod crustacea callinectes latimanus (Rathbun) and cardisoma armatum (herklots)
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Preparative and analytical thin layer Chromatography have been used to purify and separate phospholipids from the total lipids of Callinectes latimanus (Decapoda: Portunidae) and Cardisoma armatum Decapoda: Gecarcinidae). Comparison of Rf values of standards with standard Rf values in the solvent system employed indicated the presence of phosphatidy 1 ethanolamine (PE; Rf = 0.61), phosphatidy 1 choline (PC; Rf =03.34), phophatidic acid (PA; Rf = 0.76) and phosphatidy 1 – N-methy 1 ethanolamine (PNME; Rf = 0.85). An unidentified spot with Rf = 0.93 was also detected. Gas liquid chromatography of the total lipid extract from the two Crustaceans indicated that the predominant saturated fatty acid in the two Crustaceans is palmitic acid (C16:0) with oleic acid (C18:1) occurring as the major unsaturated fatty acid. The probable role of phosphatidyl – N –methyl ethanolamine and the composition of the fatty acid methyl esters (FAME) are discussed.
- Technical Report