Molecular genetic monitoring of bacterial communities in manzala lake, egypt, based on 16S rRNA gene analysis
El Saied, H.E.
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A first molecular genetic study on the diversity of bacterial communities at Manzala Lake, Egypt, was determined by culture-independent 16S rRNA gene analysis. Bulk DNAs were extracted from water and sediment at two different sampling sites namely; Bashtir and Genka, in the lake. The 16S rRNA gene was positively amplified by polymerase chain reaction (PCR) from bulk DNA of each sample, cloned and sequenced. The sequence analysis of one hundred clones from each clone library obtained number of phylotypes ranged from 8 phylotypes, in Genka water, to 20 phylotypes in each of the sediment samples. The 16S rRNA gene showed diversity in sediment samples higher than in water samples. Based on Libshuff pairwise clone library comparisons, the 16S rRNA gene phylotypes from Bashtir water encompassed the phylotypes of Genka water, while the phylotypes of Genka water account only a portion of those of Bashtir water. Both water and sediment samples were dominated by phylotypes closely related to dif.....