Gene bank preparation from the endemic shrimps and crustaceans of Persian Gulf and Oman Sea
Tamadoni Jahromi, Saeid
Rezvali Gilkolaei, S.
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Genetic knowledge and Gene bank preparation can help to protect biodiversity and detect , species identify , fishing offenses , genetic classification and also identification the faliure cross hybridizations of marine animals . In this study, sampling was performed from Jask, guatr and Hormuz areas, which is the most important habitats for the species studied using bottom trawl. Total DNA extraction was performed using phenol - chloroform method. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic.....
PublisherIranian Fisheries Science Research Institute